Research Assistant (Pharmacy and Pharmaceutical Sciences)

Interested applicants are invited to apply directly at the NUS Career Portal. Please note your application will only be processed if you apply via NUS Career Portal.

NUS Career Portal link: https://careers.nus.edu.sg/job/Research-Assistant-%28Pharmacy-and-Pharmaceutical-Sciences%29/32038-en_GB/

We regret that only shortlisted candidates will be notified.

Job Description

The successful candidate will work with Dr. Boxiang Liu on the wetlab validations of AI-optimised mRNA sequences for stability and protein expression.

The main responsibilities of the position include:
- Gene synthesis and in vitro mRNA transcription for transfection into cells.
- Cell line construction and maintenance.
- Analysis of protein expression by flow cytometry.
- High throughput sequencing of gene libraries.
- Characterisation of mRNA vaccine and therapeutic effects in vivo.

Qualifications

Qualifications / Discipline:
- Bachelor’s or Master's degree in molecular biology, cell biology, immunology, or a related discipline; equivalent research experience will also be considered.  

Skills: 
- Proficiency in PCR, cloning, mammalian cell culture and transfection, fluorescence microscopy and immunostaining is required.
- Experience with fluorescence-activated cell sorting and flow cytometry data analysis, in vitro transcription, high throughput gene library construction and sequencing, and mouse model perturbation and analysis is advantageous.
- Excellent self-motivation, teamwork skills, and the ability to design and troubleshoot experiments are preferred.

Experience: 
- Prior laboratory experience in molecular biology, cellular biology or immunology projects with mammalian cell experiments.
- Experience with in vivo experiments, particularly with mouse models, is an advantage.
- Contribution to at least one paper in a relevant field. Having a published first author paper is an advantage.